Open AccessSimultaneous analysis of eprosartan and hydrochlorothiazide in tablet formulation by High- Performance thin layer chromatography with ultraviolet absorption densitometry
Author(s) -
Vrushali Tambe,
Vijaya Vichare,
Anupa Wagh,
Surekha Wavhal
Publication year - 2021
Publication title -
international journal of pharmaceutical chemistry and analysis
Language(s) - English
Resource type - Journals
eISSN - 2394-2797
pISSN - 2394-2789
DOI - 10.18231/j.ijpca.2021.024
Subject(s) - hydrochlorothiazide , densitometry , chromatography , thin layer chromatography , chemistry , acetic acid , materials science , analytical chemistry (journal) , medicine , blood pressure , biochemistry
A new, simple, accurate, and precise high-performance thin-layer chromatographic method has been established for simultaneous analysis of Eprosartan and Hydrochlorothiazide from a tablet formulation. Standard and sample solutions of Eprosartan and Hydrochlorothiazide were applied to precoated 250 μm layer of silica gel G 60 F and the plates were developed with Chloroform: Acetonitrile: Glacial Acetic Acid (7:3:1,v/v/v) as mobile phase. Detection and evaluation of densitograms was performed densitometrically at 254 nm. The linear range was 200-700 ng/band with the retention factors of Eprosartan and Hydrochlorothiazide were 0.26± 0.02 and 0.44±0.02, respectively. The method was validated and successfully used for analysis of the drugs in tablets.