Open AccessDevelopment of novel HPTLC method for determination of imidazole antifungal drug fenticonazole: Exploring hydrotropy
Author(s) -
Minakshi D. Pawara,
Snehal Pardeshi,
Amol R Gaware,
Amod S. Patil,
Manoj V Girase
Publication year - 2021
Publication title -
international journal of pharmaceutical chemistry and analysis
Language(s) - English
Resource type - Journals
eISSN - 2394-2797
pISSN - 2394-2789
DOI - 10.18231/j.ijpca.2021.016
Subject(s) - miconazole nitrate , chemistry , chromatography , nitrate , toluene , silver nitrate , silica gel , analytical chemistry (journal) , materials science , nuclear chemistry , antifungal , microbiology and biotechnology , miconazole , organic chemistry , biology
A novel High-Performance Thin-Layer Chromatography (HPTLC) method was portrayed for the determination of Fenticonazole Nitrate (FTZ) in Bulk and Vaginal Capsules. The estimation of Fenticonazole Nitrate was achieved on aluminium pre-coated sheets of silica gel 60 F(10 cm × 10 cm) using mobile phase Toluene: Methanol: Triethylamine (4:1:0.5 v/v/v). Densitometry detection of Fenticonazole Nitrate was performed at 254nm. Fenticonazole nitrate demonstrated a strong correlation with a coefficient of correlation of 0.999 over the concentration range of 500 – 3000 ng/band. The Rvalue for Fenticonazole Nitrate was found to be 0.65. As per International Conference on Harmonization the established method was successfully validated to various parameters like accuracy, precision, sensitivity, specificity, robustness and shows the satisfactory results for all parameters. The recognized method is simple, accurate, precise, robust, sensitive and economical in nature. This method can be used for quality control analysis of Fenticonazole Nitrate in bulk and vaginal capsules.