Open AccessObservational study of Y chromosome microdeletion, EAA markers and non EAA markers in Chhattishgarh
Author(s) -
Michael S. Sinha,
Suprava Patel,
Nilaj Bagde,
Human Prasad Sinha,
Apoorva Joshi
Publication year - 2021
Publication title -
indian journal of obstetrics and gynecology research
Language(s) - English
Resource type - Journals
eISSN - 2394-2754
pISSN - 2394-2746
DOI - 10.18231/j.ijogr.2021.066
Subject(s) - y chromosome microdeletion , infertility , male infertility , y chromosome , medicine , genetics , multiplex polymerase chain reaction , offspring , biology , gynecology , gene , pregnancy , polymerase chain reaction
Genetic factors contribute to 15% of all causes of male infertility. Y chromosome microdeletion is the second most common genetic cause of male infertility. Screening is important for Yq microdeletion as the defect can be transferred to offspring. Aim of our study is to detect the frequency of Y chromosome microdeletion in idiopathic infertile men using both EAA and non EAA markers in central region of India. Forty men from infertility clinic, seeking treatment of infertility were recruited in the study as cases. Thirty normal fertile men of same origin were recruited as controls. Semen analysis was done and cytogenetic normal infertile men were included in the study. Simplex and multiplex PCR methods were used to detect Yq microdeletions. Frequency of deletion was 11/40 (27.5%). Single deletion of AZF a,b,c were 12.5%, 7.5%, 2.5% respectively (). Double deletions of AZF a+c and b+c were 2.5% each (). Two subjects showed deletion for more than one loci. Overall frequency of deletion depends on sample size, no of markers used, inclusion criteria of subjects and geographic location. So, the screening is important for Yq microdeletion as the defect may be inherited to offspring.