Open AccessEstimation of urinary delta aminolevulinic acid levels in gasoline and petrol pump workers as an index of lead exposure
Author(s) -
Sachin A. Patharkar,
Neelam J. Patil,
Siddhesh Thorat,
Alka V. Nerurkar,
Umesh R. Shinde,
Laxman Rama Chougule,
Jalinder Sanap,
Kalpana Shinde
Publication year - 2022
Publication title -
international journal of clinical biochemistry and research
Language(s) - English
Resource type - Journals
eISSN - 2394-6377
pISSN - 2394-6369
DOI - 10.18231/j.ijcbr.2021.058
Subject(s) - levulinic acid , urine , gasoline , urinary system , amino acid , chemistry , biochemistry , food science , biology , organic chemistry , endocrinology , catalysis
Lead poisoning is a phenomenon which with growing globalization is being a subject of worry.ALA i.e Amino levulinic acid is a precursor of hemoglobin, which is synthesized in mitochondria by two main components succinyl Co-A and glycine in presence of ALA-S i.e. amino levulinic acid-synthase. Urinary ALA (ALA-U) has been a recommended biomarker for lead exposure. Inhibition of Amino levulinic acid-dehydratase (ALA-D) results into activation of ALA-S which further synthesizes ALA, excess of ALA is accumulated in the blood, plasma, urine. Present manuscript is focused on the estimation of levels of ALA in the urine of gasoline and pertol pump workers, by acidifying the urine to extract out ALA and reading it colorimetrically as they are exposed to fumes released by gasoline, petrol, and petroleum products which contains lead. Awareness and safety measures such as protective masks and gears should be provided by the respective organisations to the workers.