Open AccessMultivariate analytical methods for simultaneous estimation of Atenolol and Hydrochlorothiazide in bulk and tablet dosage form
Author(s) -
Sreenivasa Charan Archakam,
Keerthisikha Palur,
Praveen Kumar Arava
Publication year - 2021
Publication title -
ip international journal of comprehensive and advanced pharmacology/ip international journal of comprehensive and advanced pharmacology
Language(s) - English
Resource type - Journals
eISSN - 2581-5555
pISSN - 2456-9542
DOI - 10.18231/j.ijcaap.2021.037
Subject(s) - hydrochlorothiazide , atenolol , dosage form , chemistry , chromatography , linear regression , matrix (chemical analysis) , multivariate statistics , mathematics , analytical chemistry (journal) , statistics , medicine , blood pressure
The present study aimed to develop simple, accurate and precise FTIR and UV spectrophotometric methods for the quantification of Atenolol and Hydrochlorothiazide in bulk and tablet dosage forms. FT-IR method like classical least squares (CLS) was developed within the range of 2366.69-3433.44; 1564.40-1673.30 cm- UV methods like Cramer’s matrix method (method-I) and linear regression analysis (Method II) were developed and they are based upon constructing the matrix set by using molar absorptivity values at 275.60 nm and 270.40 nm. The assay values for FTIR- CLS method were 102% and 108 % for Atenololand Hydrochlorothiazide respectively. Cramer’s matrix method results were found to be 95.15% and 104% for Atenolol and Hydrochlorothiazide respectively and for linear regression method they were found to be 98.50% and 106% (w/w).