Open AccessSuitability of Papanicolaou stain for micronuclei mass screening in buccal smears comparing reference feulgen stain and papanicolaou stain in field
Author(s) -
Suresh Amin,
Nilam Patel,
Bharat B. Chattoo
Publication year - 2018
Publication title -
international journal of molecular and immuno oncology
Language(s) - English
Resource type - Journals
eISSN - 2836-3779
pISSN - 2456-3994
DOI - 10.18203/issn.2456-3994.intjmolimmunooncol20184341
Subject(s) - papanicolaou stain , feulgen stain , stain , papanicolaou test , staining , micronucleus test , medicine , pathology , cancer , cervical cancer , toxicity
Background: Micronuclei (MN) assessment so far was done directly, individually, and intra- and, here, inter-observer bias could not be eliminated. It was subjective. Methods: In this study, accurate and reproducible MN counting was facilitated by the use of liquid cytology and image capturing with MN identification analysis. Results: Comparing the two nuclear staining techniques - DNA specific Feulgen stain and commonly available Papanicolaou (PAP) stain we found that both techniques gave statistically comparable accurate and reproducible MN results. T-test and non-parametric followed by paired non-parametric and then Wilcoxon matched-pairs signed rank test tell us that both the staining procedures have no difference at P <0.05. Conclusion: These protocols are comparable and reproducible, and may be adoptable for mass screening to assess the genotoxic effects of substances like tobacco.