Open AccessStudy of stability of proline-containing derivatives of dopamine and serotonin in the biological media in vitro experiments
Author(s) -
К. В. Шевченко,
Л. А. Андреева,
I. Yu. Nagaev,
В. П. Шевченко,
N. F. Myasoedov
Publication year - 2019
Publication title -
biomedicinskaâ himiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.192
H-Index - 15
eISSN - 2310-6972
pISSN - 2310-6905
DOI - 10.18097/pbmc20196506498
Subject(s) - carboxypeptidase , proline , chemistry , aminopeptidase , dopamine , serotonin , in vitro , biochemistry , biological activity , endopeptidase , stereochemistry , leucine , enzyme , amino acid , biology , endocrinology , receptor
Boc-Gly-Pro-DP, Z-Gly-Pro-DP, LA-Gly-Pro-DP, Boc-Gly-Pro-Srt, Z-Gly-Pro-Srt were synthesized for the first time. The stability of these compounds in the presence of leucine aminopeptidase, carboxypeptidase Y, carboxypeptidase B and proline endopeptidase (PEP) was determined. It turned out that the compounds are stable in the presence of aminopeptidases and carboxypeptidases. In the presence of PEP, dopamine (DP) and serotonin (Srt) are cleaved from the synthesized preparations. Thus, new proline-containing Srt and DP derivatives were obtained, Srt and DP could be gradually released from them. This suggest the possibility of a prolonged action of these biologically active compounds on the vital activity of cells and, consequently, of the whole organism.