Open AccessSensitivity of Extracellular Vesicles from Human Blood Serum to Various Detergents
Author(s) -
A. A. Yakovlev,
Т. А. Дружкова,
Alla Guekht,
N. V. Gulyaeva
Publication year - 2020
Publication title -
biomedical chemistry: research and methods
Language(s) - English
Resource type - Journals
ISSN - 2618-7531
DOI - 10.18097/bmcrm00143
Subject(s) - vesicle , chemistry , chaps , chromatography , pulmonary surfactant , size exclusion chromatography , sodium dodecyl sulfate , microvesicles , bromide , sepharose , micelle , extracellular , solubilization , membrane , ether , cationic polymerization , biophysics , biochemistry , enzyme , biology , organic chemistry , aqueous solution , microrna , gene
Blood exosomes and microvesicles, collectively known as small extracellular vesicles (sEV), are vesicles about 100-150 nm in size. Small EV are involved in various aspects of signaling in the body; in addition, they can serve as markers of various pathologies. For biochemical studies, vesicle solubilization is often required. We tested the ability of various detergents to dissolve membranes of the sEV. Small EV were isolated from the blood serum of healthy volunteers by gel filtration on Sepharose CL-2B and tried to solubilize them using the anionic detergent DOC (sodium deoxycholate), non-ionic detergent Brij 35 (polyoxyethyleneglycol dodecyl ether), zwitterionic detergent CHAPS (3 - [(3-chloramidopropyl) dimethylammonio] -1-propanesulfonate), and cationic detergent CTAB (cetyl trimethylammonium bromide). The concentration of sEV in the solution was determined by dynamic light scattering. We find DOC is the most effective for sEV solubilization.