Open AccessApplication of the SPR Biosensor in Drug Prototypes Discovery with Human Cytochrome P450(51) as an Example
Author(s) -
Leonid Kaluzhskiy,
П. В. Ершов,
Tatsiana Shkel,
O. V. Gnedenko,
Natalia V. Ivanchina,
Natallia Strushkevich,
A. A. Kicha,
Irina Grabovec,
А. А. Гилеп,
Sergey A. Usanov,
Valentin A. Stonik,
А. С. Иванов
Publication year - 2018
Publication title -
biomedical chemistry: research and methods
Language(s) - English
Resource type - Journals
ISSN - 2618-7531
DOI - 10.18097/bmcrm00055
Subject(s) - surface plasmon resonance , biosensor , computer science , drug discovery , cytochrome p450 , computational biology , biochemical engineering , selection (genetic algorithm) , field (mathematics) , nanotechnology , combinatorial chemistry , chemistry , materials science , engineering , machine learning , biology , biochemistry , mathematics , metabolism , nanoparticle , pure mathematics
The development of the integral platform “From Gene to Lead”, consolidated computer methods, bioinformatics researches, and experimental approaches, significantly accelerated and optimized base structure search in the field of drug design. The necessity of the experimental verification of hundreds virtual structure hypothesis (results of molecular data base selections or de novo construction) requires demands the usage of the high-through out and sensitive methods for validation possible interaction between numerous of selected compounds and particular molecular targets and evaluation of affinity, kinetics and thermodynamics. Surface plasmon resonance (SPR) technology makes it possible to solve all these problems. In this article the methodical aspects of the optical SPR-biosensor usage in the field of drug prototypes selection are described using the human cytochrome P450(51) catalyzing one of the key step of cholesterol biosynthesis as an example.