Open AccessDevelopment of 3D Cell Culture on Ultra-High Molecular Weight Polyethylene (UHMWPE) as the Basis of Cellular Matrix
Author(s) -
Aleksey A. Ustyugov,
Maria M. Chicheva,
Ekaterina A. Lysikova,
E. A. Vikhareva,
N. A. Sipyagina,
Ale. Malkova,
Elena A. Straumal,
Elena Bovina,
Ф.С. Сенатов,
Alexey I. Salimon,
Aleksey V. Maksimkin,
С. А. Лермонтов
Publication year - 2018
Publication title -
biomedical chemistry: research and methods
Language(s) - English
Resource type - Journals
ISSN - 2618-7531
DOI - 10.18097/bmcrm00048
Subject(s) - polyethylene , ultra high molecular weight polyethylene , matrix (chemical analysis) , microbiology and biotechnology , extracellular matrix , cell culture , intracellular , materials science , in vitro , biophysics , chemistry , biology , composite material , biochemistry , genetics
The study is devoted to the development of an artificial material based on the ultrahigh-molecular weight polyethylene (UHMWPE) with a porous or cellular 3D structure as a cellular matrix – a framework for growing cell cultures. The development of such matrix provides support for neuronal cell culture under conditions that mimick those that exist in the living body. Typically, in vitro cellular studies are conducted in a 2D format, which limits intercellular interactions, morphology, differentiation, survival, signaling responses, gene expression and proliferation that are found in vivo. Here, we propose to use UHMWPE as a material of the cellular matrix, the ultra-high molecular weight polyethylene. UHMWP is a bioinert substance, wich allows forming a system of open connected pores needed to provide cellular life conditions with supply of nutrients and oxygen as well as the removal of waste products, the possibility of intercellular communication, etc. As a result, the use of UHMWPE as a cellular matrix will allow to study the processes occurring in cells in the 3D environment.