Evaluation of Antibacterial Activity and Phytochemical Constituents of Leaf Extract of Lippia adoensis

There are quite large numbers of traditionally used medicinal plants that are used to treat skin disorder in the ethno medical system of Ethiopia. Medicinal plants namely L. adoensis, was screened for antibacterial activity against different strains of bacteria which are known to cause various types of skin infections and food poisoning Anti bacterial effect of the plant species was evaluated against different bacterial strains. The leaves of plant species were extracted by maceration and soxhelt extraction technique for preparation of crude and fractional extract respectively. And anti bacterial screening of different concentration of both crude and fractional extract of the plant species were determined using agar well diffusion method. The test organisms were one gram positive (S.aures) and three gram negative (Salmonella typhi, E.coli and P.aeruginosa) standard organisms. The results of the initial antibacterial screening test indicated the potential of these herbal drugs in treating bacterial infections of the skin and food poisoning. Among the different fractions (petroleum ether, chloroform, acetone and methanol) tested for antibacterial activity, the non-polar fractions were found to be more active than the polar fractions. The Phytochemical screening tests carried out on L. adoensis indicated the presence of tannins, flavonoids and saponins. Different extracts L.adoensis were showed significant antibacterial activity against the S.aueres, P.aeroginosa, E.coli and S.typhi. Hence further study is recommended to identify the specific active ingredient and potential formulation of effective antibiotic.