Open AccessSTABILITY OF THIOAMIDE TYPE OF PIPERINE UNDER ACIDIC AND BASIC CONDITIONS
Author(s) -
Nurul Hikma,
Risfah Yulianty,
Muhammad Aswad
Publication year - 2021
Publication title -
journal of experimental biology and agricultural sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.108
H-Index - 2
ISSN - 2320-8694
DOI - 10.18006/2021.9(spl-2-icopmes_2020).s259.s263
Subject(s) - piperine , thioamide , chemistry , chromatography , acetonitrile , potency , high performance liquid chromatography , aqueous solution , stereochemistry , organic chemistry , biochemistry , in vitro
Thioamide type of piperine, “thiopiperine” is a derivate of piperine that having high potency against breast cancer cells. This research was intended to evaluate the stability of thiopiperine under highly acidic and basic conditions for 72 h at 60⁰C. This study was conducted by the SHIMADZU® UFLC system integrated with a PDA detector, while the analysis was performed in an isocratic separation mode using column C-18 (COSMOSIL®), 150 mm x 4.6 mm, column particle size: 5 µm. Chromatography condition was set using a mobile phase consisting of 50% aqueous acetonitrile with a flow rate of 1 mL/min, while the detection wavelength was 340 nm. The result showed that thiopiperine undergoes extensive degradation under acidic and basic environments.