Open AccessAnalisis Keragaman Gen Laktoferin Pada Sapi Friesian-Holstein Dengan Metode Pcr-Rflp
Author(s) -
Elmy Mariana
Publication year - 2011
Publication title -
jurnal agripet
Language(s) - English
Resource type - Journals
eISSN - 2460-4534
pISSN - 1411-4623
DOI - 10.17969/agripet.v11i1.649
Subject(s) - lactoferrin , genotyping , restriction fragment length polymorphism , genotype , ecori , restriction enzyme , biology , polymorphism (computer science) , gene polymorphism , microbiology and biotechnology , gene , genetics , veterinary medicine , medicine
Polymorphism analysis of lactoferrin gene on holstein-friesian cow with PCR-RFLP methodABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene in Holstein-Friesian (HF) cows. The study was conducted on 281 heads of HF lactating cows coming from dairy farmers in Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65.5%) and AB (34.5%) genotypes.