Open AccessFusarium incarnatum: A Novel Leaf Spot Pathogen Isolated from Rosa indica in Pakistan
Author(s) -
Shazia Shafique
Publication year - 2021
Publication title -
international journal of agriculture and biology/international journal of agriculture and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.271
H-Index - 39
eISSN - 1814-9596
pISSN - 1560-8530
DOI - 10.17957/ijab/15.1768
Subject(s) - biology , pathogen , leaf spot , inoculation , fusarium , spots , pathogenicity , fungus , crop , botany , fungal pathogen , virulence , horticulture , agronomy , microbiology and biotechnology , gene , biochemistry
Fungal leaf spots disease is a main reason of rose (Rosa indica L.) crop failure in Pakistan. Isolation and identification of pathogen from rose plant was done by observing phenotypic characters which were further executed on molecular bases using ITS, EF and Bt2a/Bt2b primers. On these bases, pathogen was identified as Fusarium incarnatum. Then, Koch’s pathogenicity test was applied to confirm the virulence level of the isolated fungus by artificially inoculating it on R. indica seedlings in plate and pot trials. This study signifies the first report of F. incarnatum as a leaf spot pathogen of R. indica in Pakistan and highlights the need to explicate the management strategies of pathogen populations. © 2021 Friends Science Publishers