Open AccessHeterogeneous Expression of Cymbidium longibracteatum Magnesium Protoporphyrin IX Methyltransferase (ClChlM) Activates Chlorophyll Biosynthesis in Transgenic Tobacco
Author(s) -
Yu Jiang
Publication year - 2021
Publication title -
international journal of agriculture and biology/international journal of agriculture and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.271
H-Index - 39
eISSN - 1814-9596
pISSN - 1560-8530
DOI - 10.17957/ijab/15.1746
Subject(s) - nicotiana benthamiana , chloroplast , biology , cymbidium , biosynthesis , chlorophyll , biochemistry , transgene , open reading frame , protoporphyrin ix , gene , protochlorophyllide , ectopic expression , botany , peptide sequence , chemistry , photodynamic therapy , organic chemistry
Magnesium protoporphyrin IX methyltransferase (ChlM) plays an important role in the regulation of chlorophyll biosynthesis and chloroplast development. In the present study, we isolated a ChlM gene, designated ClChlM, from Cymbidium [Cymbidium longibracteatum (Wu & Chen) Chen & Liu]. The open reading frame (ORF) sequence of ClChlM was 945 bp and encoded a putative protein of 314 amino acids. The deduced ClChlM contained the conserved SAM/SAH binding pocket and substrate binding sites. Subcellular localization analysis of ClChlM revealed that the protein was localized in the chloroplast. Ectopic overexpression of ClChlM in tobacco (Nicotiana benthamiana Domin) increased ALA-synthesizing capacity and chlorophyll content and widely upregulated the expression level of photosynthesis-related genes, such as ClHemA, ClGSA, ClLhcb, ClCHLI, and ClCHLH. In conclusion, these results demonstrate that ClChlM plays a crucial role in the regulation of chlorophyll biosynthesis in C. longibracteatum and will help in breeding for leaf colour variance in the future. © 2021 Friends Science Publishers