Open AccessWatermelon Chlorotic Stunt Virus is Associated with Cucumber Yellow Mosaic Symptoms in Oman
Author(s) -
Muhammad Shahid,
Muhammad Shafiq,
Abdullah M. Al-Sadi
Publication year - 2021
Publication title -
international journal of agriculture and biology/international journal of agriculture and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.271
H-Index - 39
eISSN - 1814-9596
pISSN - 1560-8530
DOI - 10.17957/ijab/15.1739
Subject(s) - cucumis , begomovirus , biology , phylogenetic tree , cucurbitaceae , cloning (programming) , genome , dna sequencing , cucumber mosaic virus , melon , virology , plant virus , dna , botany , virus , horticulture , genetics , gene , computer science , programming language
Cucumber (Cucumis sativus; family Cucurbitaceae) plants exhibiting begomovirus-like symptoms such as yellowing, mosaics and stunting were studied using cloning, sequencing, Species Demarcation Tool followed by phylogenetic clustering. The complete genome of DNA-A showed maximum sequence identity of 98.7% with the corresponding DNA-A of an isolate from “Iran” strain of Watermelon chlorotic stunt virus (WmCSV). The DNA-B displayed 97.5% nt identity with the component of DNA-B of WmCSV from Iran, too. Our results confirmed that yellowing and mosaic symptoms of cucumber are associated with a bipartite begomovirus (WmCSV). This study is the first characterization of WmCSV in association with described symptoms in cucumber from Oman. © 2021 Friends Science Publishers