Open AccessGenome-Wide Identification and Expression Analysis of the NRAMP Family in Melon
Author(s) -
Lan Jin
Publication year - 2021
Publication title -
international journal of agriculture and biology/international journal of agriculture and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.271
H-Index - 39
eISSN - 1814-9596
pISSN - 1560-8530
DOI - 10.17957/ijab/15.1672
Subject(s) - biology , gene , gene family , abscisic acid , biochemistry , arabidopsis , genetics , arabidopsis thaliana , signal transduction , methyl jasmonate , jasmonate , transmembrane domain , cucumis , genome , botany , mutant
The NRAMPs (natural resistance-associated macrophage proteins) are evolutionarily conserved integral membrane proteins that transport a broad range of metal ions. A comprehensive analysis of NRAMP family genes has not been reported for melon (Cucumis melo). In this study, six CmNRAMP genes were identified from the melon genome. Analyses of gene structure, phylogeny, chromosome location and conserved motifs revealed two groups of CmNRAMPs: one group contained only CmNRAMP5, which had 12 conserved motifs like NRAMPs in Arabidopsis thaliana and rice (Oryza sativa). The other group contained five CmNRAMPs and all had shorter amino acid sequences compared with CmNRAMP5. Expression analyses suggested that CmNRAMP genes are expressed differently in response to treatments with different plant hormones, abiotic factors, and metals (iron, copper, and manganese). The most responsive gene was CmNRAMP5, which showed significant changes in its transcript levels in response to abscisic acid (ABA), indole acetic acid, cytokinin, methyl jasmonate, brassinosteroid, Fe, and Cu. The least responsive gene was CmNARMP6, which was only significantly responsive to ABA, Fe, and Mn. These results indicate that CmNRAMP genes function in numerous tissues and at different developmental stages. A protein–protein interaction analysis indicated that CmNRAMPs interact with proteins involved in transmembrane transport, superoxide dismutase activity, plant hormone signal transduction, signal transduction by protein phosphorylation, and nicotianamine synthase activity. This study provides valuable insights into the potential function of CmNRAMP genes and their encoded products. © 2021 Friends Science Publishers