Open AccessIsolation and Identification of Endophytic Bacteria from the Roots of the Endangered Plant Ostrya rehderiana
Author(s) -
Xiang TaiHe,
Tong XiaXiu,
Wang KeYun,
Wang LeiZhen
Publication year - 2020
Publication title -
international journal of agriculture and biology/international journal of agriculture and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.271
H-Index - 39
eISSN - 1814-9596
pISSN - 1560-8530
DOI - 10.17957/ijab/15.1261
Subject(s) - biology , paenibacillus , paenibacillus polymyxa , bacteria , rhizobium , botany , agrobacterium , pseudomonas , bacillus (shape) , endangered species , endophyte , 16s ribosomal rna , microbiology and biotechnology , gene , genetics , ecology , transformation (genetics) , habitat
Ostrya rehderiana Chun, one of the most endangered species in China mainly because of difficult transplantation, is distributed in Western Tianmu Mountain of the Zhejiang Province in China. There are presently only five plants still remaining in the wild. Since plant endophytes affect the growth and development of the host plant either directly or indirectly, in this study, we adopted the tissue block separation method to isolate 22 endophytic bacteria strains from the roots of O. rehderiana, with 10 strains from wild O. rehderiana and 12 from O. rehderiana plantations. From morphological analysis and 16S rDNA sequence analysis, 6 known genera were identified from the 22 strains. Among the isolated strains, eight were Bacillus (the dominant endophytic bacteria in the root of O. rehderiana), six were Paenibacillus, three were Agrobacterium, three were Paraburkholderia, one was Rhizobium, and one was Pseudomonas. Both wild and plantation O. rehderiana contained Bacillus, Paenibacillus and Paraburkholderia, which presumably could promote the growth of O. rehderiana. Besides, the Rhizobium strain isolated from wild O. rehderiana might be beneficial to the growth of O. rehderiana, but the Agrobacterium strains from the plantations might impair the growth of O. rehderiana. © 2020 Friends Science Publishers