In Vitro Evaluation of the Composite Alginate - Calcium Phosphate Materials for Prototyping Technologies in Bone Defects Substitution | Zendy

Н. С. Сергеева | Zendy; В. С. Комлев | Zendy; И. К. Свиридова | Zendy; В. А. Кирсанова | Zendy; С. А. Ахмедова | Zendy; Е. А. Кувшинова | Zendy; A. Yu. Fedotov | Zendy; A. Yu. Teterina | Zendy; С. М. Баринов | Zendy

Open Access

In Vitro Evaluation of the Composite Alginate - Calcium Phosphate Materials for Prototyping Technologies in Bone Defects Substitution

Author(s) -

Н. С. Сергеева,

В. С. Комлев,

И. К. Свиридова,

В. А. Кирсанова,

С. А. Ахмедова,

Е. А. Кувшинова,

A. Yu. Fedotov,

A. Yu. Teterina,

С. М. Баринов

Publication year - 2015

Publication title -

n n priorov journal of traumatology and orthopedics

Language(s) - English

Resource type - Journals

eISSN - 2658-6738

pISSN - 0869-8678

DOI - 10.17816/vto201522128-34

Subject(s) - gelatin , sodium alginate , composite number , mtt assay , calcium alginate , adhesive , chemistry , cytotoxicity , matrix (chemical analysis) , in vitro , calcium , materials science , chemical engineering , sodium , biomedical engineering , composite material , biochemistry , organic chemistry , layer (electronics) , medicine , engineering

Porous granular composite biomaterials based on sodium alginate and calcium phosphate component: 1% sodium alginate; sodium alginate with gelatin (80/20); calcium phosphate ceramics (CHA, TCP and OCP) and composite materials alginate/CHA, alginate/TCP, alginate/OCP, alginate/gelatin (80/20)/TCP and alginate/TCP/CHA/OCP were investigated for cytotoxicity and matrix (for cells) properties in dynamics of cultivation (up to 21 days). Two adhesive cell lines - immortalized human fibroblasts (HF, strain 1608 hTERT) and human osteosarcoma (MG-63) were used for in vitro MTT assay. OCP material showed its cytotoxicity while all its composites possessed weak matrix properties. For further research on the development of prototyping technologies 3 samples of composite materials were selected: alginate/CHA, alginate/TCP and alginate/gelatin (80/20)/TCP that are characterized by cytocompatibity and the presence of either expressed or moderate matrix properties of the surface for cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research