Open AccessStudy of Biocompatibility and Antibacterial Properties of Argentum-Tricalcium Phosphate In Vivo
Author(s) -
V. E Khon,
N. V Zagorodniy,
Vasily Mamonov,
Е Н Гласко,
Н. В. Петракова,
A. N. Shal’Nev,
T. Ya. Pkhakadze,
V. C. Komlev
Publication year - 2014
Publication title -
vestnik travmatologii i ortopedii imeni n.n. priorova
Language(s) - English
Resource type - Journals
eISSN - 2658-6738
pISSN - 0869-8678
DOI - 10.17816/vto20140356-61
Subject(s) - biocompatibility , in vivo , femur , antibacterial activity , bone marrow , subcutaneous tissue , in vitro , phosphate , chemistry , biomedical engineering , materials science , nuclear chemistry , medicine , surgery , pathology , biology , bacteria , biochemistry , organic chemistry , genetics , microbiology and biotechnology
Study of biocompatibility and antibacterial properties of argentum-containing tricalcium phosphate (TCPh) with various degree of substitution by argentum (TCPh-Ag) was performed on rat model. Biocompatibility was studied using subcutaneous implantation of TCPh-Ag (0.5). Antibacterial properties were studied in 5 subgroups (A-E), 5 animals in every group. At first step a distal femur defect was formed and infected by 24-hours cultures of clinical isolates of methicillin-resistant S. aureus. Second step (subgroups B-E) consisted of revision intervention with implantation of either TCPh or TCPh-Ag with various degree of substitution by argentum (0.04, 0.2 and 0.5) into infected defect. Histologic examination of tissue samples with subcutaneous implantation of TCPh-Ag (0.5) showed the absence of inflammatory and rejection reactions that was indicative of study material biocompatibility. Optimim degrees of Ag substitution were 0.2 and 0.5 that provide high antibacterial activity at monotherapy of femur bacterial infection in rats with minimum pronounced toxic effect on the surrounding tissues and bone marrow.