Open AccessRooting of <i>Vaccinium corymbosum</i> L. microshoots cv. «Blue-Berry» in culture <i>in vitro</i> and <i>ex vitro</i>
Author(s) -
Gamil El-Dis Mohamed,
Л. З. Хуснетдинова,
О. А. Тимофеева
Publication year - 2018
Publication title -
samarskij naučnyj vestnik
Language(s) - English
Resource type - Journals
eISSN - 2782-3016
pISSN - 2309-4370
DOI - 10.17816/snv201874114
Subject(s) - vaccinium , subculture (biology) , berry , acclimatization , zeatin , shoot , micropropagation , botany , explant culture , horticulture , biology , cytokinin , in vitro , auxin , biochemistry , gene
This paper discusses a method of micropropagation of Vaccinium corymbosum L. cv. Blue-Berry. The results showed that WPM supplemented with 1,0 mg/l of zeatin in combination with 0,1 mg/l of indolyl-3-butyric acid was more effective for the multiplication of blueberry axillary shoots. The maximum increase in the number of healthy axillary shoots was observed in the fourth subculture, whereas the phenomenon of hyperhydration (vitrification) began to appear in the fifth subculture. In addition, it was established that the presence of indolyl-3-butyric acid and 1,0 g/l of activated charcoal in the nutrient medium lead to the development of good root system of the Vaccinium corymbosum cv. Blue-Berry. The highest mean number of roots formed per explant was obtained on WPM medium, supplemented with 0,5 mg/l indolyl-3-butyric acid after 10 weeks. Acclimatization of in vitro regenerated plantlets of Vaccinium corymbosum with a developed root system in ex-vitro conditions (pH 3,54) showed a 100% survival rate.