Open AccessChanges of intracellular potentials and ionic currents of the mollusk neurons under action of solvents dimethylsulfoxide and acetone
Author(s) -
А. И. Вислобоков,
К. Н. Мельников,
I. N. Tyurenkov,
Petr Dmitriyevich Shabanov
Publication year - 2014
Publication title -
obzory po kliničeskoj farmakologii i lekarstvennoj terapii
Language(s) - English
Resource type - Journals
eISSN - 2542-1875
pISSN - 1683-4100
DOI - 10.17816/rcf12110-14
Subject(s) - depolarization , acetone , chemistry , hyperpolarization (physics) , biophysics , extracellular , electrophysiology , microelectrode , voltage clamp , sodium , neuron , ion , ionic bonding , membrane potential , intracellular , biochemistry , stereochemistry , organic chemistry , neuroscience , biology , electrode , nuclear magnetic resonance spectroscopy
Dimethylsulfoxide (DMSO), and dimethyl ketone (acetone) in concentrations up to 100 mM after extracellular application did not change the electrical activity of the identified pedal ganglia neurons of clam Planorbarius corneus in microelectrode studies and voltage clamp technique. DMSO in concentrations of 500 and 1000 mM (4 and 8 % solution), and acetone in smaller concentrations (from 200 mM-1.2% or higher) was shown to depolarize neurons and to increase the frequency and duration of action potentials, their amplitude and the total ionic currents (dV/dt) being decreased. The changes were fully reversible, hyperpolarization of cells was registered after washing, which was caused probably by activation of electrogenic transport of sodium ions. DMSO and acetone were suggested to change the neuron state not only by changes in resting potential but also by direct effects on ion channels. Acetone reduced amplitude of action potentials in neurons because of depolarization and in less degree because of its effects on ion channels. DMSO and acetone in concentrations of 100-200 mM are concluded to be safe for biological objects when they are used for dissolution of pharmacological agents.