Open AccessDesign of ELISA test-kits for organ specific autoantibodies determination on the base of microsome fractions of adrenal and gonads steroid producing cells
Author(s) -
Platon P. Khokhlov,
Хохлов Платон Платонович,
Petr Dmitriyevich Shabanov,
Шабанов Петр Дмитриевич
Publication year - 2012
Publication title -
obzory po kliničeskoj farmakologii i lekarstvennoj terapii
Language(s) - English
Resource type - Journals
eISSN - 2542-1875
pISSN - 1683-4100
DOI - 10.17816/rcf10483-90
Subject(s) - autoantibody , gonad , microsome , antibody , medicine , immune system , antigen , endocrinology , endocrine system , immunology , biology , in vitro , biochemistry , hormone
The aim of this work was to design ELISA test-kits for quantitative measurement of autoantibodies to immune reactive structures of human steroid-producing cells. The antigens were immobilized in solid phase including microsome fractions of human adrenals, Leidig male cells and female ovarian granulose cells. The use of biopsy of human adrenal and gonad material, standardized antibodies fractions of patients with adrenal or gonad autoimmune lesion, have permitted to design ELISA test-kits. That test-kits have high sensibility and specificity to organ-specific autoantibodies for autoimmune diseases. These test-kits on the bases of microsome fractions of human adrenals and gonads as immobilized antigens have sensibility, accuracy and precision needed for such routine ELISA kits.