Open AccessDynamics of pertussis antibodies in the saliva of children revaccinated with DPT vaccine
Author(s) -
N F Amfiteatrova,
A. O. Kiselev
Publication year - 1989
Publication title -
kazanskij medicinskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2587-9359
pISSN - 0368-4814
DOI - 10.17816/kazmj99912
Subject(s) - saliva , agglutination (biology) , antibody , medicine , polystyrene , latex fixation test , virology , immunology , chemistry , organic chemistry , polymer
One of the promising methods that can be used in the rapid diagnosis of pertussis is the latex agglutination reaction (RLA), which we developed based on the platelet stained latex microagglutination reaction (RPOLMA). The aim of this work was to investigate the possibility of determining pertussis antibodies in the saliva of children revaccinated with DPT-vaccine by RPLMA. Polystyrene monodisperse latex with particle diameter of 0.55 m, prepared in the All-Russian Research Institute of Synthetic Rubber (Leningrad) and sensitized with pertussis bacteria disintegrator containing 775 g/ml protein (according to Lowry) in the ratio 1 : 2, was used as antigen for RPOLMA.