Open AccessA rapid microscopic examination of tissues. Dudgeon and Patrick (Ref. Zentr. f. Chir., 1928, No. 26)
Author(s) -
I. Tsimkhes
Publication year - 1928
Publication title -
kazanskij medicinskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2587-9359
pISSN - 0368-4814
DOI - 10.17816/kazmj95783
Subject(s) - distilled water , alcohol , acetic acid , clearance , chemistry , chromatography , absolute (philosophy) , mercury (programming language) , nuclear chemistry , organic chemistry , medicine , philosophy , epistemology , computer science , urology , programming language
The authors take thin pieces of tissue freshly cut with a sharp scalpel and immerse them for 2 or, better, 10 minutes in Schandin's solution (2 parts saturated solution of mercury chloride, 1 part of absolute alcohol, a few drops of vinegar so that the solution contains 4% acetic acid. absolute alcohol and a few drops of vinegar so that the solution contains 4% acetic acid solution), then the preparation is washed in alcohol and distilled water, stained with Mauer's haemalaun and eosin, dried and cleared with absolute alcohol and xylene, after which the sections are embedded in Canadian balsam.