Cultivation of gonococci. Mityukevich (Microb. J., 1928, vol. 1-2)

Mityukevich convinced that the best medium for growing gonococci is made from fresh, never sterilized before meat water and martin peptone: this environment is added (by Ikoma) citric acid in the amount necessary to lower the Ph from 8.0 to 7.8, the mixture is sterilized at 115 in a 20-30 minute, and 10% ascitic fluid is added to it, the suitability of which for this purpose has been verified by preliminary experiments; to obtain solid media, 1.7% agar is added to the broth. The best growth of gonococci on this medium is obtained at 36.