Open AccessDetermination of streptococci in blood in sepsis. Verger (Deut. med. Woch., 1924, No. 20)
Author(s) -
В. И. Сергеев
Publication year - 1925
Publication title -
kazanskij medicinskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2587-9359
pISSN - 0368-4814
DOI - 10.17816/kazmj80217
Subject(s) - pipette , erlenmeyer flask , agar plate , sepsis , whole blood , medicine , microbiology and biotechnology , chemistry , chromatography , biology , surgery , bacteria , genetics
In almost all cases of chronic sepsis, it was possible to prove the presence of streptococci in the blood by the following method: 5-10 cc of blood is collected in a sterile test tube, waiting for the blood to clot, after which the serum is drained, and the blood clot is transferred to an Erlenmeyer flask containing 50 cc. 10% horse serum broth. The flask is placed in the thermostat for 4 to 6 days, with a few drops taken from the bottom with a sterile capillary pipette every 24 h and transferred to the blood agar plate.