Open AccessDetermination of papilloma-virus infection reproductive phase in algorithm of monitoring patients with cervical displasia
Author(s) -
S. A. Selkov,
G. N. Vedeneeva,
I. A. Baskakova,
Siegfried Baur
Publication year - 2021
Publication title -
žurnalʺ akušerstva i ženskihʺ boleznej
Language(s) - English
Resource type - Journals
eISSN - 1684-0461
pISSN - 1683-9366
DOI - 10.17816/jowd91125
Subject(s) - medicine , cervical intraepithelial neoplasia , immunocytochemistry , cervical cancer , hpv infection , polymerase chain reaction , human papilloma virus , virus , virology , gynecology , cancer , biology , gene , biochemistry
HPV 16 and 18 are known to be the main cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The terms of HPV persistence in the host and, coordinately, the risk of cervical neoplasia development and progression are determined in much extent by virus activity. The purpose of this investigation was the detection of HPV DNA presence in cervical epithelium as well as confirmation of its activity by means of immunocytochemistry and reverse transcriptase polymerase chain reaction. The level of HPV inf ection by oncogenic and nononcogenic types in 181 women with different cervical pathology was 55,8%. The active stage of HPV infection was confirmed in 27,5% of HPV-inf ected women mainly with low grades of CIN. The proof of reproductive general HPV infection was more informative with RT PCR just as for HPV 16 and 18 immunocytochemistry and RT PCR completed each anothe.