About obtaining a clean distribution of Neisser's gonococci using the plate method

Trapped several times on a platinum needle, particles of gonorrhea pus from the urethra or from the contents of the tubes with ascending gonorrhea are thoroughly loosened in liquid human blood serum and then prepared from the latter two dilutions according to a known method. Tubes with seeded particles are placed in a water bath at 40 C, and their contents are mixed with approximately equal volume of diluted agar (20% agar, 1% peptone, 0.5% NaCl) and poured onto a plate. The plates are placed in a humid chamber and placed in a thermostat at 36-37 C.