Open AccessComparison of laboratory methods for diagnosis of Trichomonas vaginalis
Author(s) -
Zinaida M. Martikaynen,
Aleksey N. Grigoryev,
Olga Sergeyevna Ryzhkova,
Yelena Vasilyevna Shipitsyna,
Alevtina Savicheva,
И. В. Литвиненко,
Alla Valentinovna Galkina,
Nina Igorevna Kudryavtseva,
Yelena Mikhaylovna Tangatarova,
Olga Aleksandrovna Lomakina,
Yelena Petrovna Protasova,
Svetlana Yuryevna Romantseva
Publication year - 2014
Publication title -
žurnalʺ akušerstva i ženskihʺ boleznej
Language(s) - English
Resource type - Journals
eISSN - 1684-0461
pISSN - 1683-9366
DOI - 10.17816/jowd6315-9
Subject(s) - trichomoniasis , trichomonas vaginalis , isolation (microbiology) , trichomonas , medicine , trichomonas vaginitis , microbiology and biotechnology , liquid culture , pathology , biology , gynecology , botany
The paper presents data on comparison of microscopy, culture and molecular (PCR) methods for diagnosis of trichomoniasis, as well as on evaluation of the quality of culture media for isolation of trichomonads produced by Russian and international manufacturers. The highest sensitivity was shown for the molecular (real-time PCR) method. Microscopy of stained and wet smears demonstrated relatively high sensitivity. The culture media that are widely used in our country for Trichomonas vaginalis isolation need optimization.