Open AccessINDEL-GENOTYPING OF VIBRIO CHOLERAE STRAINS
Author(s) -
A. S. Vodop’Yanov,
Водопьянов Алексей Сергеевич,
С. О. Водопьянов,
Водопьянов Сергей Олегович,
Igor Paulovich Oleynikov,
Олейников Игорь Павлович,
Б Н Мишанькин,
Мишанькин Борис Николаевич
Publication year - 2017
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40978
Subject(s) - vibrio cholerae , genotyping , genotype , biology , cholera , indel , cholera toxin , genetic diversity , genetics , isolation (microbiology) , microbiology and biotechnology , population , gene , bacteria , single nucleotide polymorphism , medicine , environmental health
The aim of this work was to genotype Vibrio cholerae strains, based on 9 INDEL-markers. We identified 26 genotypes in 223 strains studied. Based on the cluster analysis, all genotypes were grouped into 8 clusters. A geographic attachment to specific regions was characteristic for some of the clusters. Toxigenic strains formed a separate cluster and have genotypes different from atoxigenic strains. All strains V cholerae O1 Eltor, isolated from 1929 to 2014, had an identical genotype, different from the genotypes of V. cholerae O1 classical and V. cholerae O139. A loss of cholera toxin gene was shown to fail to give rise in the alteration of INDEL-genotype. This allows us recommend this genotyping method for the identification strains that had a cholera toxin gene in the past. Under annual isolation of strains of V. cholerae from environmental objects, their population was established to be particular due to a high level of genetic diversity. This makes it possible to propose the use of the diversity index as one of the criteria for the assessment of epidemiological risk.