Open AccessMonoclonal antibodies to thermostable surface antigens of Vibrio cholerae O1 and O139 serogroups
Author(s) -
V. V. Evdokimova,
Л. П. Алексеева,
О. Ф. Кретенчук,
В. Д. Кругликов,
И. В. Архангельская,
О. С. Бурша
Publication year - 2015
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40893
Subject(s) - vibrio cholerae , epitope , monoclonal antibody , antigen , biology , microbiology and biotechnology , antibody , el tor , cholera , genotype , gene , bacteria , genetics
There is delivered the panel of 9 hybridomas producing monoclonal antibodies directed to surface thermo-resistant antigenic determinants of V.cholerae El Tor and O139. By immunoblotting method most of MCA were established to react with the corresponding epitopes of protein nature at the level of 38-42 kDa. There were revealed differences in the specific orientation of the MCA to Vibrio cholerae: the first group of MCA identifies antigenic determinants presented only in strains of V. cholerae O1, O139 with genetic characteristic ctx+ tcp+ and ctx- tcp+, but fails to identify strains ctx- tcp-; the second group of MCA identifies common epitopes for V.cholerae El Tor and O139 regardless of ctx and tcp genes. The new panel of MCA can be used as well for the study of the fine antigenic structure of V. cholerae El Tor and O139 as detection of epidemically dangerous strains genotype ctx+ tcp+ with ELISA methods