Open AccessExamination of convalescents after West Nile fever for the specific IgM, IgG and neutralizing antibodies
Author(s) -
Alla R. Azaryan,
Anna Kozlova,
А. П. Гришанова,
E. I. Ivashchenko,
Г Л Шендо,
A. I Kovtunuv,
В. А. Антонов,
Д. В. Викторов,
V. P. Smelyansky,
Е. А. Снатенков,
И А Баркова,
В. Ф. Ларичев,
А. М. Бутенко
Publication year - 2016
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40890
Subject(s) - titer , serology , virology , antibody , medicine , west nile virus , specific antibody , immunology , virus
The examination was executed in the Astrakhan region over 2013-2014 with participation of three laboratories. In 26 convalescents after West Nile Fever (WNF) with the serological confirmed diagnosis of WNF blood sera were examined. Neurological form of WNF was observed in 8 cases, and febrile form - in 8 convalescents. Sera have been collected in 243-358 days, on average, in 308 days after the beginning of a disease. For their examination there were used IFA-IgM ELISA (MAC-ELISA), IgG ELISA methods as well as neutralization test in Vero E6 cell culture. The results of the examination in 24 of 26 patients (92, 3%) for IgM antibodies to the virus WNF were negative. In two convalescents according to the laboratories in Astrakhan and Moscow in sera there were observed low titers of IgM (1:400) with minor indices of sera optical density (0.3 to 0.4) and negative results in the Volgograd Plague Control Research Institute. Serum samples of other two convalescents were weakly positive or questionable in testing in Volgograd, but were negative when examined in the Institute of Virology. Specific IgG antibodies were detected in 23 of 26 convalescents (88.5%), neutralizing in 22 of 24 (91.7 %). These data confirm the adequacy of the criteria and tactics for WNF serodiagnosis adopted in Russia based on the application of the MAC -ELISA (IFA - IgM).