Open AccessMethod of DNA-comet assay in the diagnosis of liver fibrosis in patients with chronic viral hepatitis C
Author(s) -
А. Ф. Попов,
Anna Mikhailov,
Наталя Іванова,
А. И. Симакова
Publication year - 2015
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40855
Subject(s) - comet assay , peripheral blood , fibrosis , dna damage , viral hepatitis , medicine , chronic hepatitis , gastroenterology , liver fibrosis , dna , liver damage , immunology , pathology , virology , biology , virus , biochemistry
In the study there were included 100 patients, which were selected in 5 group in dependence on the degree of liver fibrosis: I - F0, II - F1, III - F2, IV- F3, V - F4. Sixth (control) group was consisted of 43 healthy volunteers. The performed research has shown the relationship between the stage of fibrosis and the degree of DNA degradation in peripheral blood lymphocytes. The biggest level of DNA destruction in peripheral blood lymphocytes was established in group V patients with chronic viral hepatitis C. This allowed to consider method of DNA-comet assay as promising auxiliary method in the assessment of the formation of liver fibrosis.