MALDI-ToF mass spectrometry analysis with molecular genetic identification of Vibrio spp. in the system of the monitoring of vibrio flora of surface water reservoirs | Zendy

Л. В. Миронова | Zendy; E. A. Basov | Zendy; М. В. Афанасьев | Zendy; Zh. Yu. Khunkheeva | Zendy; С К Миткеева | Zendy; V. S. Ganin | Zendy; L. Ya. Urbanovich | Zendy; Е. С. Куликалова | Zendy; E. G. Goldapel | Zendy; С. В. Балахонов | Zendy

Open Access

MALDI-ToF mass spectrometry analysis with molecular genetic identification of Vibrio spp. in the system of the monitoring of vibrio flora of surface water reservoirs

Author(s) -

Л. В. Миронова,

E. A. Basov,

М. В. Афанасьев,

Zh. Yu. Khunkheeva,

С К Миткеева,

V. S. Ganin,

L. Ya. Urbanovich,

Е. С. Куликалова,

E. G. Goldapel,

С. В. Балахонов

Publication year - 2014

Publication title -

epidemiology and infectious diseases

Language(s) - English

Resource type - Journals

eISSN - 2411-3026

pISSN - 1560-9529

DOI - 10.17816/eid40838

Subject(s) - vibrio , rpob , vibrio cholerae , microbiology and biotechnology , biology , 16s ribosomal rna , flora (microbiology) , mass spectrometry , identification (biology) , bacterial taxonomy , bacteria , chromatography , chemistry , ecology , genetics

There is presented the assessment of the efficacy of the application of direct protein profiling on the basis of MALDI-ToF mass-spectrometry for identification of Vibrio spp. during monitoring of Vibrio flora in surface water reservoirs implemented in the network of cholera surveillance. The comparison of results of the MALDI-ToF MS and bacteriological detection of taxonomic belonging of583 colonies morphologically similar to Vibrio cholerae (isolated in bacteriological examination of samples from environmental objects in Irkutsk city in 2012-2013) with following random identification based on 16S rRNA and rpoB gene structure showed high diagnostic sensitivity and specificity of mass-spectrometry. The findings determine the expediency of the inclusion of MALDI-ToF MS in the layout of microbiological examination in the monitoring of Vibrio flora in surface water reservoirs.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research