Open AccessMALDI-ToF mass spectrometry analysis with molecular genetic identification of Vibrio spp. in the system of the monitoring of vibrio flora of surface water reservoirs
Author(s) -
Л. В. Миронова,
Е. А. Басов,
М. В. Афанасьев,
Zh. Yu. Khunkheeva,
С К Миткеева,
V. S. Ganin,
L. Ya. Urbanovich,
Е. С. Куликалова,
E. G. Goldapel,
С. В. Балахонов
Publication year - 2014
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40838
Subject(s) - vibrio , rpob , vibrio cholerae , microbiology and biotechnology , biology , 16s ribosomal rna , flora (microbiology) , mass spectrometry , identification (biology) , bacterial taxonomy , bacteria , chromatography , chemistry , ecology , genetics
There is presented the assessment of the efficacy of the application of direct protein profiling on the basis of MALDI-ToF mass-spectrometry for identification of Vibrio spp. during monitoring of Vibrio flora in surface water reservoirs implemented in the network of cholera surveillance. The comparison of results of the MALDI-ToF MS and bacteriological detection of taxonomic belonging of583 colonies morphologically similar to Vibrio cholerae (isolated in bacteriological examination of samples from environmental objects in Irkutsk city in 2012-2013) with following random identification based on 16S rRNA and rpoB gene structure showed high diagnostic sensitivity and specificity of mass-spectrometry. The findings determine the expediency of the inclusion of MALDI-ToF MS in the layout of microbiological examination in the monitoring of Vibrio flora in surface water reservoirs.