Open AccessTHE INFLUENCE OF SOME FACTORS ON THE FORMATION OF BIOFILM BY TOXIGENIC AND NON-TOXIGENIC VIBRIO CHOLERAE EL TOR STRAINS
Author(s) -
О. А. Татаренко,
Л. П. Алексеева,
Н Р Телесманич,
И. С. Шестиалтынова,
O. C. Chemisova,
О. В. Маркина,
N. B. Nepomnyashchaya,
Н. Н. Ускова
Publication year - 2012
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40714
Subject(s) - vibrio cholerae , microbiology and biotechnology , biofilm , vibrio , el tor , biology , polystyrene , microtiter plate , chemistry , bacteria , genetics , organic chemistry , polymer
The early stages of biofilm formation on polystyrene surface were studied in 14 Vibrio cholerae El Tor strains, all of which carried genes of vps cluster, mshA, and hapR genes. 13 Vibrio cholerae El Tor strains were shown to be capable of adsorption on the walls of microtiter plate wells and of ring formation at the "polystyrene-medium" interface. One of the Vibrio cholerae El Tor strains tested was defective in this ability. The use of different media - modified M1 medium and 1 per cent peptone - failed to reveal sufficient influence of culture medium composition on the ability of Vibrio cholerae to form multilayer biofilm at the phase boundary "polystyrene-medium". Temperature reduction from 37°C to 25°C also didn t result in statistically significant enhancement of monolayer formation in both media. Quantitative evaluation of biofilm formation showed that this ability was clearly marked only in two toxigenic and two non-toxigenic strains of Vibrio cholerae El Tor. Monolayer comparison in ELISA demonstrated that only two toxigenic and two non-toxigenic strains possessed the ability for exopolysaccharide production.