Open AccessSEROTYPING OF STREPTOCOCCUS PNEUMONIAE STRAINS, ISOLATED FROM CHILDREN IN URAL REGION WITH THE USE OF MULTIPLEX PCR
Author(s) -
Е. В. Саматова,
A. E. Druy,
Grigory Tsaur,
Л. Г. Боронина
Publication year - 2012
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.17816/eid40701
Subject(s) - serotype , streptococcus pneumoniae , microbiology and biotechnology , multiplex polymerase chain reaction , pneumococcal infections , virology , medicine , biology , polymerase chain reaction , antibiotics , gene , genetics
This article presents results of the multiplex PCR investigation of the serotypes distribution of S. pneumoniae strains circulating in Ekaterinburg and the Sverdlovsk region. This study was performed in children with invasive, noninvasive pneumococcal infections and carriers. 118 strains of pneumococci typed out of 129 ones (91.5%) referred to the 15 serotypes: 6A, 6B (20,8%); 23F (13,9%); 19F (11,5%); 8, 9V, 9A, 11F, 11A, 11B, 11C, 11D, 12F, 15A, 33F (11,5%); 3 (10%) 2, 15F, 17F, 22F, 23B (3,9%); 18B, 18C (3.9%), 19A (3,2%); 7F, 19B, 19C, 23A (3,2%); 5,10A (1.6%), 20 (1.6%), 14 (1, 6%); 9L, 9N, 15B, 15C (1,6%); 18F (1,6%); 18A (1.6%). Coincidence rate of serotypes S. pneumoniae, isolated from children with chronic infectious and inflammatory diseases of the lung with serotypes included into the content of the conjugate vaccines is: 7-valent - 69.3%, 10-valent - 98.2%, 11 - and 13-valent - 100%.