Open AccessComparative cytochemical analysis of hydrogen peroxide distribution in pea ineffective mutant SGEFix--1 (sym40) and initial line SGE
Author(s) -
Anna V. Tsyganova,
V. E. Tsyganov,
Aleksey U Borisov,
И. А. Тихонович,
N. J. Brewin
Publication year - 2009
Publication title -
èkologičeskaâ genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.148
H-Index - 3
eISSN - 2411-9202
pISSN - 1811-0932
DOI - 10.17816/ecogen733-9
Subject(s) - hydrogen peroxide , mutant , bacteria , matrix (chemical analysis) , biology , chemistry , catalase , microbiology and biotechnology , oxidative stress , biochemistry , gene , genetics , chromatography
Comparative cytochemical analysis has revealed differences in hydrogen peroxide distribution in symbiotic nodules of pea initial line SGE and mutant SGEFix - -1 (sym40). In the initial line SGE, precipitates of cerium perhydroxide were deposited in the walls of infection threads and in adjacent material in the luminal matrix. In mutant SGEFix - -1, an increased deposition of cerium perhydroxide precipitates was observed in the matrix of hypertrophied infection droplets, round bacteria contained in infection threads and also around juvenile bacteroids. The observed pattern of hydrogen peroxide distribution indicates that bacteria in infected cells of mutant nodules are exposed to a stronger oxidative stress compared with nodules of the initial line.