Open AccessINTERACTION OF RECOMBINANT TATA-BINDING PROTEIN WITH MAMMALS GENES PROMOTER TATA
Author(s) -
Л. К. Савинкова,
И. А. Драчкова,
М. П. Пономаренко,
М. В. Лысова,
T. V. Arshinova,
Nikolay А. Kolchanov
Publication year - 2007
Publication title -
èkologičeskaâ genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.148
H-Index - 3
eISSN - 2411-9202
pISSN - 1811-0932
DOI - 10.17816/ecogen5244-49
Subject(s) - tata box , tata binding protein , recombinant dna , tata box binding protein , gene , microbiology and biotechnology , biology , promoter , oligonucleotide , genetics , gene expression
Quantitative characteristics of interaction recombinant TATA-binding protein (TBP) with oligonucleotides identical to natural TATA-containing promoter region genes of mammals are received. In particular, new experimental data about the importance guanine in 8-th position of the TATA-element for affinity to TBP are received. The experimental data, testifying that raised maintenance G and С nucleotides in flanks of TATA-element does the contribution to affinity to TBP are received.