Open AccessUniversal 16s rRNA Primers BD1 For Soil Microbial Community Analysis
Author(s) -
Elisaveta V Korostik,
А. Г. Пинаев,
Gulnar A. Akhtemova,
Е. Е. Андронов
Publication year - 2006
Publication title -
èkologičeskaâ genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.148
H-Index - 3
eISSN - 2411-9202
pISSN - 1811-0932
DOI - 10.17816/ecogen4432-37
Subject(s) - 16s ribosomal rna , biology , ribosomal rna , genetics , gene , clone (java method) , microbiology and biotechnology
New universal 16S rRNa primers were constructed and tested. These primers allow identifying correct taxonomic position of bacterial isolates and were shown to be useful in microbial community studies. The primers enable to detect the vast majority of unique 16S rRNa gene sequences. In the study 160 restriction types were found in 16S rRNa clone library (190 clones).