Open AccessSomaclonal variability of conifers in culture in vitro
Author(s) -
Elena Gulyaeva,
Roman V. Ignatenko,
Н. А. Галибина
Publication year - 2020
Publication title -
èkologičeskaâ genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.148
H-Index - 3
eISSN - 2411-9202
pISSN - 1811-0932
DOI - 10.17816/ecogen19143
Subject(s) - somaclonal variation , somatic embryogenesis , biology , tissue culture , botany , callus , somatic cell , in vitro , genetics , gene
The use of somatic embryogenesis is one of the promising methods of conifer propagation on an industrial scale. However, this technology has a number of problems, which include the appearance of somaclonal variation in cell and tissue culture. The review considers the causes and methods for detecting somaclonal variability of conifer in culture in vitro. It is shown that it is necessary to use a complex of molecular, cytogenetic, morphological, physiological methods for the analysis of somaclonal changes in embryogenic plant cultures.