Open AccessExperimental evaluation of the effectiveness of local treatment of skin burns with сarbopol easier to disperse 2020 hydrogel with human leukemia differentiation factor-6 peptide
Author(s) -
E. V. Zinoviev,
Vasiliy N. Tsygan,
Ilya V. Artsimovich,
M S Asadulayev,
M B Paneyakh,
V V Zubov,
S. A. Lukyanov,
D. V. Kostyakov,
A A Zhilin,
Ю. М. Лопатин,
A A Umanskaya,
К. Ф. Османов,
S N Kravtsov,
Vasiliy Ya. Apchel,
Yu. A. Zolotarev,
A. K. Dadayan,
K A Dadayan
Publication year - 2018
Publication title -
vestnik rossijskoj voenno-medicinskoj akademii
Language(s) - English
Resource type - Journals
eISSN - 2687-1424
pISSN - 1682-7392
DOI - 10.17816/brmma12319
Subject(s) - peptide , leukemia , granulation tissue , wound healing , human skin , leukemia inhibitory factor , extracellular matrix , medicine , chemistry , inflammation , immunology , biology , biochemistry , interleukin 6 , genetics
The results of experimental evaluation of the effectiveness of topical application of the peptide human leukemia differentiation factor-6 in carbopol hydrogel easier to disperse 2020 in thermal burns of skin of III degree are presented. It was found that local application of human leukemia differentiation factor-6 peptide in hydrogel carbopol in concentration from 0,01% to 0,00001% causes acceleration of wound epithelialization processes by 28 days by 71,11% (p0,01) and reduces the incidence of purulent complications by 62,5% (p0,05). Histological examination of biopsy specimens of wounds revealed essential features of morphometry, considering the chosen method of treatment and concentration of peptide of human leukemic factor-6 differentiation in carbopol hydrogel. The proposed method of local treatment of burn skin lesions is very promising for optimizing the flow of reparative regeneration processes. The low frequency of purulent complications in the group of animals treated with a minimum peptide content may be due to the combined action of carbopol hydrogel as determinants of wound healing in a wet environment, and the optimal concentration of the peptide human leukemia differentiation factor-6, providing anti-inflammatory effect in a minimum concentration. Analysis of the histological pattern in the studied groups of animals allows us to conclude about the acceleration of the formation of the extracellular matrix, and epithelialization of the wound surface on the background of an application of the peptide human leukemia differentiation factor-6 minimum concentration. On the 14th day of observation the number of microvessels in granulation tissue among animals treated with human leukemia differentiation factor-6 in carbopol hydrogel easier to disperse 2020 with minimum concentration (0,00001%) was 40,6-43,75% more than in the comparison groups. The use of biologically active peptides is one of the promising methods of wound process optimization. human leukemia differentiation factor-6 peptide can selectively stimulate proliferation by enhancing redox processes in cells.