
Enhancing the stability of bentgrass (AgrostisstoloniferaL.) to salts of cadmium
Author(s) -
Evgeny Aleksandrovich Gladkov,
О. Н. Гладкова,
L. S. Glushetskaya
Publication year - 2012
Publication title -
izvestiâ mgtu "mami"
Language(s) - English
Resource type - Journals
eISSN - 2949-1428
pISSN - 2074-0530
DOI - 10.17816/2074-0530-68472
Subject(s) - cadmium , callus , chemistry , botany , horticulture , biology , organic chemistry
In the paper there is developed a biotechnological method for increasing the stability of urban turf – bentgrass, to ions of cadmium in the environment. The following scheme was chosen selection – culturing callus on MS medium supplemented with 10 mg Cd / l for two passages, regeneration on MS medium supplemented with 20 mg Cd / l, rooting on MS medium with cadmium concentration of 30 mg / l. Under selective conditions there were received 130 regenerants of bentgrass, 58 of which were rooted in the soil. Resistance to cadmium was kept at one of the seeds tested regenerants in four generations.