Open AccessProcess of production Аgrostis stolonifera l. plants, resistant to high concentrations of lead via cell engineering
Author(s) -
Evgeny Aleksandrovich Gladkov,
О. Н. Гладкова,
L. S. Glushetskaya,
Yu. I. Dolgikh
Publication year - 2014
Publication title -
izvestiâ mgtu "mami"
Language(s) - English
Resource type - Journals
eISSN - 2949-1428
pISSN - 2074-0530
DOI - 10.17816/2074-0530-67560
Subject(s) - agrostis stolonifera , callus , lead (geology) , botany , pulp and paper industry , horticulture , chemistry , biology , poaceae , engineering , paleontology
The technology of production Agrostis stolonifera lawn grass resistant to salts of lead was developed. For the production of plants tolerant to lead the direct selection circuit was used. This circuit compromises culturing the callus over two passages in the modified MS medium with 650 mg/l of lead, regeneration and rooting plants on MS medium with 650 mg/l of lead. The obtained plants remained high decorative quality at high concentrations of lead and may be used as starting shapes upon receipt of varieties tolerant to this metal.