Open AccessQuantitative determination of water-soluble polysaccharides in the aerial part of <i>Potentilla anserina L.</i>
Author(s) -
Ekaterina S. Tyutrina,
Е. Е. Савельева,
Vladimir I. Kurbatsky
Publication year - 2020
Publication title -
aspirantskij vestnik povolžʹâ
Language(s) - English
Resource type - Journals
eISSN - 2410-3764
pISSN - 2072-2354
DOI - 10.17816/2072-2354.2020.20.3.191-196
Subject(s) - potentilla , polysaccharide , extraction (chemistry) , raw material , yield (engineering) , chemistry , botany , grinding , chromatography , biology , materials science , biochemistry , organic chemistry , metallurgy , composite material
The article presents the gravimetric technique which was developed for the quantitative determination of water-soluble polysaccharides (WSPS) in the aerial part of Potentilla goose (Potentilla anserina).
The purpose of the work was to develop the method of the quantitative determination of WSPS and the determination of their content in the aerial part of Potentilla anserina L., collected in various regions of Siberian federal district.
Materials and methods. The object of the study was the aerial part of Potentilla anserina L, collected in June during flowering. To develop the method for the quantitative determination of the WSPS, herb mixtures collected in 2016 in vil. Verkhneussinskoe of Ermakovsky district, Krasnoyarsk Territory were studied. The amount of WSPS was determined in the above-ground part of Potentilla anserina L., collected during flowering in June 2017 in the Krasnoyarsk Territory (vil. Sliznevo), Tomsk Region (Tomsk Region), and the Republic of Buryatia (Zaigraevsky District, vil. Staraya Bryan). The above-ground parts were air dried in the shade. Determination of water-soluble polysaccharides was carried out gravimetrically in order to determine the optimal conditions for the maximum yield of water-soluble polysaccharides There was some variety in the degree of grinding of raw material, raw material / extractant index, extraction ratio, the ratio of extraction and ethyl alcohol 95%.
Results. The greatest amount of water-soluble polysaccharides was extracted in the following conditions: the degree of grinding of raw material of 0.5-1.0 mm; the ratio of raw material and extractant was 1 : 10; the extraction rate was 5; the ratio of extraction and ethyl alcohol 95% was 1 : 4. The content of water-soluble polysaccharides was 7.55 0.36%, the analysis error did not exceed 5%.
Conclusion. A gravimetric technique for the quantitative determination of water-soluble polysaccharides in the aerial part of P. anserina L has been developed. The content of water-soluble polysaccharides in the aerial part of P. anserina L. collected in various regions of the Siberian Federal District ranges from 5.95% (Krasnoyarsk Region) to 10.25% (Republic of Buryatia), the analysis error does not exceed 5%.