Open AccessUltrasound-Assisted Emulsified Microextraction Based on Deep Eutectic Solvent for Trace Residue Analysis of Metribuzin in Urine Samples
Author(s) -
Mehran Pourhossein,
Omid Reza Heravizadeh,
Fariborz Omidi,
Monireh Khadem,
Seyed Jamaleddin Shahtaheri
Publication year - 2021
Publication title -
metody i obʺekty himičeskogo analiza
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.138
H-Index - 3
eISSN - 2413-6166
pISSN - 1991-0290
DOI - 10.17721/moca.2021.153-161
Subject(s) - chromatography , detection limit , deep eutectic solvent , calibration curve , solvent , reproducibility , extraction (chemistry) , chemistry , residue (chemistry) , materials science , eutectic system , analytical chemistry (journal) , biochemistry , organic chemistry , alloy
Deep eutectic solvent was used as extraction solvent to develop and optimize a new sample preparation method for the determination of metribuzin in urine samples. In order to determine the optimal values of the effective factors in the deep eutectic solvent-based ultrasound assisted emulsification microextraction method, six effective parameters were selected. The design of experiments was performed using the onevariable- at-a-time method. Totally, 96 experimental runs were performed, and the samples were analyzed using high-performance liquid chromatography with a UV detector. Under the optimum conditions, the calibration curve for metribuzin was linear in the concentration range of 5 to 500 μg L-1 for urine samples. The accuracy and reproducibility of the introduced method were determined using the relative recovery (RR %) and relative standard deviation (RSD %) tests on the fortified urine samples. RR % and RSD % were found to be 96.3 – 101.7 % and 3.2 – 7.6 %, respectively. The limit of quantification and the limit of detection were obtained 5 and 0.8 μg L-1, respectively.