Open AccessBacterial expression of the recombinant functionally active N-terminal module of the B. taurus tyrosyl-tRNA synthetase
Author(s) -
V. N. Zayets,
O. Yu. Tsuvariev,
A. I. Kornelyuk,
L. Kolomiyets
Publication year - 2017
Publication title -
vìsnik. problemi regulâcìï fìzìologìčnih funkcìj/vìsnik kiïvsʹkogo nacìonalʹnogo unìversitetu ìmenì tarasa ševčenka. serìâ: problemi regulâcìï fìzìologìčnih funkcìj
Language(s) - English
Resource type - Journals
eISSN - 2616-6410
pISSN - 1728-2624
DOI - 10.17721/2616_6410.2017.23.33-37
Subject(s) - aminoacylation , recombinant dna , expression vector , transfer rna , microbiology and biotechnology , biology , chemistry , biochemistry , gene , rna
The nucleotide sequence coding N-terminal module of Bos taurus tyrosyl-tRNA synthetase (mini TyrRS) was cloned into the bacterial expression vector pET23d(+). Bacterial expression of the recombinant protein mini TyrRS was performed in E. coli BL21 (DE3)pLysE cells with the use of the constructed vector pET-23d(+)39YRS for subsequent physical and chemical protein studies. The catalytic activity of the recombinant mini TyrRS has been studied in the aminoacylation reaction of homologous tRNATyr.