Bacterial expression of the recombinant functionally active N-terminal module of the B. taurus tyrosyl-tRNA synthetase | Zendy

V. N. Zayets | Zendy; O. Yu. Tsuvariev | Zendy; A. I. Kornelyuk | Zendy; L. Kolomiyets | Zendy

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Bacterial expression of the recombinant functionally active N-terminal module of the B. taurus tyrosyl-tRNA synthetase

Author(s) -

V. N. Zayets,

O. Yu. Tsuvariev,

A. I. Kornelyuk,

L. Kolomiyets

Publication year - 2017

Publication title -

bulletin of taras shevchenko national university of kyiv series problems of physiological functions regulation

Language(s) - English

Resource type - Journals

eISSN - 2616-6410

pISSN - 1728-2624

DOI - 10.17721/2616_6410.2017.23.33-37

Subject(s) - aminoacylation , recombinant dna , expression vector , transfer rna , microbiology and biotechnology , biology , chemistry , biochemistry , gene , rna

The nucleotide sequence coding N-terminal module of Bos taurus tyrosyl-tRNA synthetase (mini TyrRS) was cloned into the bacterial expression vector pET23d(+). Bacterial expression of the recombinant protein mini TyrRS was performed in E. coli BL21 (DE3)pLysE cells with the use of the constructed vector pET-23d(+)39YRS for subsequent physical and chemical protein studies. The catalytic activity of the recombinant mini TyrRS has been studied in the aminoacylation reaction of homologous tRNATyr.

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