Open AccessDetergent-stable proteases from the antarctic scallop Adamussium colbecki
Author(s) -
Nataliia Raksha,
D. Gladun
Publication year - 2016
Publication title -
vìsnik. problemi regulâcìï fìzìologìčnih funkcìj/vìsnik kiïvsʹkogo nacìonalʹnogo unìversitetu ìmenì tarasa ševčenka. serìâ: problemi regulâcìï fìzìologìčnih funkcìj
Language(s) - English
Resource type - Journals
eISSN - 2616-6410
pISSN - 1728-2624
DOI - 10.17721/2616_6410.2016.20.62-65
Subject(s) - proteases , scallop , casein , plasmin , gelatin , trypsin , chemistry , biochemistry , incubation , zymography , hydrolysis , proteolysis , chromatography , enzyme , biology , fishery
Detergent-stable proteases were found in crude extract of the Antarctic scallop Adamussium colbecki. The results of gelatin zymography indicated the availability of the proteases which able to act in the presence of SDS, whereas plasmin and miniplasmin maintained their activity toward gelatin only after complete removing of detergent. Total proteolytic activity, represented by casein hydrolysis was within the control level after incubation of crude extract with detergents such as Tween 80 and SDS whereas trypsin activity was significantly reduced.