Open AccessStability of human recombinant AIMP1/Р43 protein in nanocomposite complex with beta-cyclodextrin
Author(s) -
N. V. Vorobyova,
O. Kornelyuk,
Д. М. Ложко
Publication year - 2016
Publication title -
vìsnik. problemi regulâcìï fìzìologìčnih funkcìj/vìsnik kiïvsʹkogo nacìonalʹnogo unìversitetu ìmenì tarasa ševčenka. serìâ: problemi regulâcìï fìzìologìčnih funkcìj
Language(s) - English
Resource type - Journals
eISSN - 2616-6410
pISSN - 1728-2624
DOI - 10.17721/2616_6410.2016.20.15-18
Subject(s) - nanocomposite , cyclodextrin , thermal stability , recombinant dna , linker , chemistry , materials science , nanotechnology , biochemistry , organic chemistry , computer science , gene , operating system
Stability of the recombinant AIMP1/p43 protein – component of aminoacyl-tRNA synthetase complex of higher eukaryotic – in nanocomposite complex with β-cyclodextrin was investigated. A significant increase in thermal stability AIMP1/p43 in the composition of nanocomposite complex was shown. The local conformational transition associated with the exposure of Trp271 residue on the AIMP1/p43 surface was observed at 43 0 C, but in the nanocomposite complex it was observed at 52 0 C. Stabilization of AIMP1/p43 protein in nanocomposite complex provides opportunities for further structural and functional studies and its use as a new biotechnology product in biomedicine.