Stability of human recombinant AIMP1/Р43 protein in nanocomposite complex with beta-cyclodextrin | Zendy

N. V. Vorobyova | Zendy; O. Kornelyuk | Zendy; Д. М. Ложко | Zendy

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Stability of human recombinant AIMP1/Р43 protein in nanocomposite complex with beta-cyclodextrin

Author(s) -

N. V. Vorobyova,

O. Kornelyuk,

Д. М. Ложко

Publication year - 2016

Publication title -

bulletin of taras shevchenko national university of kyiv series problems of physiological functions regulation

Language(s) - English

Resource type - Journals

eISSN - 2616-6410

pISSN - 1728-2624

DOI - 10.17721/2616_6410.2016.20.15-18

Subject(s) - nanocomposite , cyclodextrin , thermal stability , recombinant dna , linker , chemistry , materials science , nanotechnology , biochemistry , organic chemistry , computer science , gene , operating system

Stability of the recombinant AIMP1/p43 protein – component of aminoacyl-tRNA synthetase complex of higher eukaryotic – in nanocomposite complex with β-cyclodextrin was investigated. A significant increase in thermal stability AIMP1/p43 in the composition of nanocomposite complex was shown. The local conformational transition associated with the exposure of Trp271 residue on the AIMP1/p43 surface was observed at 43 0 C, but in the nanocomposite complex it was observed at 52 0 C. Stabilization of AIMP1/p43 protein in nanocomposite complex provides opportunities for further structural and functional studies and its use as a new biotechnology product in biomedicine.

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