Influence of low-intensity radiation of the optical range spectrum (λ = 630-660 nm) on the immunoresistance parameters in animals with experimental peritonitis

Treatment of inflammatory and septic conditions is a serious problem due to the existing antibiotic resistance. It is necessary to find newtreatments using biological and physical factors that affect the course of inflammatory reactions in chronic processes. It is important to understand the mechanisms of interaction of light photons with cellular acceptors, which provide reactivity and resistance.The aim – is to evaluate the low-intensity light effect of the red range spectrum (λ =630-660 nm) on the course of the inflammatory process inexperimental animals with LPS-induced peritonitis. Animals were divided into 3 groups: A – intact animals; B – animals with peritonitis induced byintraperitoneal administration of LPS; C – animals with LPS-induced peritonitis after repeated exposure to red light. Irradiation of the abdominal wall was performed with LED matrices Korobov A. – Korobov V. "Barva-Flex/24FM", which emit in the red region of the spectrum. Methods of light microscopy (study of the barrier function in oxygen-independent phagocytosis of neutrophils, estimation of the lymphocytotoxicity degree in the Terasaki test) and spectrophotometry (determination of the concentration of circulating immune complexes) were used.In animals after induction of peritonitis (group B) was observed inhibition of phagocytosis, which manifested itself in reduced adhesion andendocytosis of antigens by neutrophils compared with intact animals. The use of light exposure of the red range spectrum (group C) significantly activated phagocytic cells and reduced the degree of lymphocytotoxicity and the concentration of circulating immune complexes at different stages of the inflammatory process. Thus, the action of red light contributed to the normalization of immunoresistance in animals, thereby reducing antigenic load by activating infiltration and exudation stages of the inflammatory process and induction of regenerative processes after repeated irradiation at the end of the experiment.